FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_13YCol_Col_lib_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_13YCol_Col_lib_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1760927
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22466112.758109791036198No Hit
ATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGT72850.41370255552899127Illumina Single End PCR Primer 1 (97% over 35bp)
ACCGATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTA66120.3754840490264503Illumina Single End PCR Primer 1 (97% over 35bp)
ACATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATA39370.2235754236263059Illumina Single End PCR Primer 1 (97% over 35bp)
ACCGCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGT32840.1864926825473174Illumina Single End PCR Primer 1 (97% over 35bp)
ACGCATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTA29200.16582175183866224Illumina Single End PCR Primer 1 (97% over 35bp)
ACCGCATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCT25160.14287929028290214Illumina Single End PCR Primer 1 (97% over 35bp)
ACCAATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTA18590.10556939611920313Illumina Single End PCR Primer 1 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCATA532300.069.789482
ACCGCAT1386900.069.7513051
CCGCATT304350.069.6088942
CGCATAG120050.069.329333
CGCATAA167700.069.269423
CCGCATG238800.069.135152
CGCATAT170650.069.076953
CGCATTA77450.068.8249363
CGCATAC81950.068.804043
CCGCATC321600.068.748342
CGCATTC61100.068.052273
CGCATGG51700.068.036643
CGCATGT78850.067.913653
CGCATTG75600.067.823973
CGCATCA111450.067.707383
CGCATTT99700.067.4723053
CGCATCG46600.067.296033
CGCATGA74800.067.285993
CGCATCT114200.066.7512053
CGCATCC59650.066.655383
Produced by FastQC (version 0.11.5)