FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_13YCol_14YCol_lib_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_13YCol_14YCol_lib_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2421772
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG873813.608143128254848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGGT1491000.069.771791
CCGCATA276650.069.4907152
TGAGGTA669300.069.3714142
ACCGCAT711350.069.282181
CCGCATT156950.068.961242
TGAGGTC323200.068.893932
TGAGGTG260000.068.703542
CCGCATG125000.068.3012542
GAGGTAT217750.068.140483
GAGGTAA257100.068.07193
CGCATAA89900.067.980523
CGCATAG62150.067.6397253
GAGGTAG135100.067.3624653
CGCATAC40650.067.335823
GAGGTCT123000.067.244783
TGAGGTT263750.067.102932
CGCATAT93450.067.083583
GAGGTAC78550.067.019563
CCGCATC162400.066.646962
GAGGTCA117000.066.235643
Produced by FastQC (version 0.11.5)