FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_13YCol_14YCol_lib_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_13YCol_14YCol_lib_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2421772
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG873813.608143128254848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGGT1491700.069.768941
CCGCATA275900.069.478342
TGAGGTA669400.069.3680042
ACCGCAT710400.069.2878341
CCGCATT156700.068.983622
TGAGGTC324200.068.899082
TGAGGTG259250.068.7419742
CCGCATG124500.068.352322
GAGGTAT218600.068.11743
GAGGTAA256500.068.096363
CGCATAA90100.067.98673
CGCATAG61650.067.6222153
GAGGTAG134800.067.462133
GAGGTCT123650.067.147723
TGAGGTT263900.067.106252
CGCATAC40800.067.089933
CGCATAT93050.067.035073
GAGGTAC78550.066.9321063
CCGCATC162750.066.698862
GAGGTCA117250.066.215463
Produced by FastQC (version 0.11.5)