FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_13YCol_14YCol_15YCol_16YCol_lib_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_13YCol_14YCol_15YCol_16YCol_lib_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482558
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGA163743.3931672462170352Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG155443.221167196482081No Hit
ACTCAATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGA97492.0202752829711663Illumina Single End PCR Primer 1 (96% over 33bp)
ACTCTAATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAG13480.27934465908761225Illumina Single End PCR Primer 1 (96% over 33bp)
CTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGAT10460.21676150846115907Illumina Single End PCR Primer 1 (96% over 33bp)
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAATA7620.15790847939522296Illumina Single End PCR Primer 1 (96% over 33bp)
ATCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGAT5760.11936388993654648Illumina Single End PCR Primer 1 (96% over 33bp)
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTATGGAAAGAGTGTTAAGA5750.11915666096096221Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT97000.069.606091
CCGCATA40600.069.5647352
GTGAGGT97300.069.175641
ACTCTAT122450.068.9739761
ACCGCAT108400.068.969761
GATCGTA41550.068.816512
CGCATAG10450.068.321213
CCGCATT24050.068.24952
CCGCATG19950.068.241472
GATCGTC21300.068.188352
CGCATAC6150.067.719473
TGAGGTA42750.067.539762
CGCATAA13450.067.393683
TGAGGTG18950.067.2254642
CGCATAT12250.067.138783
GATCGTT18250.066.9274442
TGAGGTC22300.066.38612
CTCTATA60900.066.317812
GATCGTG18600.066.232542
CCGCATC25350.065.992052
Produced by FastQC (version 0.11.5)