FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_13YCol_14YCol_15YCol_16YCol_lib_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_13YCol_14YCol_15YCol_16YCol_lib_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482558
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGA163743.3931672462170352Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG155443.221167196482081No Hit
ACTCAATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGA97492.0202752829711663Illumina Single End PCR Primer 1 (96% over 33bp)
ACTCTAATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAG13480.27934465908761225Illumina Single End PCR Primer 1 (96% over 33bp)
CTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGAT10460.21676150846115907Illumina Single End PCR Primer 1 (96% over 33bp)
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAATA7620.15790847939522296Illumina Single End PCR Primer 1 (96% over 33bp)
ATCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGAT5760.11936388993654648Illumina Single End PCR Primer 1 (96% over 33bp)
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTATGGAAAGAGTGTTAAGA5750.11915666096096221Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT96850.069.605471
CCGCATA40600.069.5647352
GTGAGGT97200.069.174781
ACTCTAT122550.068.9748151
ACCGCAT108550.068.9389341
GATCGTA41550.068.816512
CGCATAG10450.068.321213
CCGCATT24050.068.24952
CCGCATG19950.068.241472
GATCGTC21300.068.188352
CGCATAC6150.067.719473
TGAGGTA42650.067.5342
CGCATAA13500.067.403313
TGAGGTG19000.067.232762
CGCATAT12200.067.127073
GATCGTT18100.066.902012
TGAGGTC22250.066.377992
CTCTATA61050.066.326852
GATCGTG18600.066.232542
CCGCATC25500.065.878352
Produced by FastQC (version 0.11.5)