FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_13YCol_14YC24_lib_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_13YCol_14YC24_lib_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9035
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4835.345877144438296No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATC1251.3835085777531821Illumina Single End PCR Primer 1 (97% over 34bp)
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTC380.42058660763696737Illumina Single End PCR Primer 1 (97% over 34bp)
ACTCAATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTC200.22136137244050913Illumina Single End PCR Primer 1 (97% over 34bp)
AGACCATGCAGCTTTGAGGCATCTGTATGCCAAGAAGGATACTAAACCAA180.19922523519645824No Hit
ATATAATTTAAGTGATACAATTAAAGCTTTTTCGATTCTTTTATTAACTG150.16602102933038185No Hit
GATATTAATAGACTCATATGGACTTCGGCTAAACCATCAATGCTTTGAGA140.1549529607083564No Hit
TTAAGACTGTTAGTATGTTTAAGCAATATATGAAGTCGATGAAGGAATCG140.1549529607083564No Hit
AATGTTAATATGAATCCTTTATTATTTCTCTCTTACGGAATTAGAATCGA140.1549529607083564No Hit
TTCAGTAATGAAACTTCTCGTTATAAAAGCAAATCTAAGAACCAGTTTCT140.1549529607083564No Hit
GTATATAATAATCACCTTCGATATCTTCTCTTGACTTATATTCGACGAGA130.14388489208633093No Hit
ATAGAAAAAAATGTTTTGTTGTAAGTACTAAAATTTTCCTATATTACTCA130.14388489208633093No Hit
TGACATCTTAATAGGAATCCGAGTGATTGCCTCGTCTTGTTCTTCTGTCA120.13281682346430548No Hit
CACATTACATCAAGTCATATTCGACTCCAAAACACTAACCAACCTTCTTC120.13281682346430548No Hit
GCGCGCACGCACTATCTTCTCTCTTTCAAAGATTGTAAAACCTAAAAGAA120.13281682346430548No Hit
TGGAACAATAAAAGATTAATTAGATGGTAGCAATCGGATTTTCACGTTTC120.13281682346430548No Hit
AGTTAATATGTTCTTCTTGCTCATAACCGGTGTGAGATGCTACAAGAAGC120.13281682346430548No Hit
TGTTTTCACGTACAGCACTCTGCTCAAGGCTTGTGTGGATGCTTCTCAGT120.13281682346430548No Hit
TGTTCTAATTATTTGAATATTCATAATACGTCTACAATTAGGATCTCTCT120.13281682346430548No Hit
AAGGTTTATATTGTTCTCGGCTATAACTCATTGTGACTCAACAAAAATTT120.13281682346430548No Hit
AATATATATAACATGCTGATTAAGTATATATGTACATTTCCTTGCCTAAT120.13281682346430548No Hit
TCGAATAATTTGCAACATTTGAGAGGGGGAGGAGGTATTTATAGCCTCCA120.13281682346430548No Hit
AAGAAGAGTGGCTTTAGGGGCCACGTAGACATTAGTTACAAAAGCTCTGC120.13281682346430548No Hit
TGAGACAATCTTGGACGCCATGGATTCACCTTAGTCTCTCTTTCTTTCAC120.13281682346430548No Hit
AGTTTTCAAATTTTTTTGTTTTTTTCTATGACTTAGATGATATGCAGACT110.12174875484228002No Hit
ATAATATTGAATGAGAGACAAGTTTAAAACTTTATATTCCATATCTGTTT110.12174875484228002No Hit
AACTGTAATAGTGGTGTAAGAACAAACGAAGCGAATATAAAAATACTTAC110.12174875484228002No Hit
ATGCTCGTTAGATAAACCTTACAATGCAAGACCATGACAACCTTAAACTC110.12174875484228002No Hit
AAAAGAAAATTGTCAACAACACGAACATATTTTAATGAAATTATTAGCAA110.12174875484228002No Hit
AAATGATCGAAGATCGGGATGGGTTTCTGTTGTCTGTTTGATTGGGATAG110.12174875484228002No Hit
AGTCTAAGTCTGGGGAGTTGATAACACGGCTTTTTAGCCCGTTTTAGCTA110.12174875484228002No Hit
ATAAGAAAGCCATAGTCAGGATATCTACACTAACCAATTCAGCATCATCG110.12174875484228002No Hit
ACCAATGTAGAGCTGGGTAGATTTTCCAGTGAGAGATTTCCATCTCCGAA110.12174875484228002No Hit
CCACGCTCATCATCGCACCAGCATTATCAAATTCACCACAGTAGTTAGGA110.12174875484228002No Hit
CCTGATAATGCTTAACTTAGATTTGTAGAATATTTTATTTGATTAGCTAA110.12174875484228002No Hit
GGTTATTTTGCTTTGAATATTTTCGGTTATTTTTAGATATTTTCGATTAT100.11068068622025456No Hit
TAGGATAATGATCTTGCAATTGCCATTACAAAGAGGAAACTTACCAGGGA100.11068068622025456No Hit
ATTCTTTAACCTGCAACGTTTGTGGTTTGTTAAGAAAACTTTATTCCACA100.11068068622025456No Hit
GATTCCTCTCATTTCCATTCCATGATGATTCCATTCGTTTCCATCCGAAG100.11068068622025456No Hit
TATCACTCTTAACATCTCCAACACTTGATTCCTCATCATTGCTTACCTTA100.11068068622025456No Hit
GAAACTAGATCTTGTAGAATCATATTGAATATTTGACGATACGTTTCGTA100.11068068622025456No Hit
GATTAAGAGATCACTGGAGTTTAGGTAAACCTTGTTCGCCGATTTAGCTA100.11068068622025456No Hit
AGAAAGAAAAATAAGAGGATCGAACCATTTCTTCTGACTCTTTTTCAAAT100.11068068622025456No Hit
TGCTGTAATCTGAAAGAACAATTTAAGCTTATATTGATGAGTTACATCTT100.11068068622025456No Hit
GACAGGGTTCCTTTCATTGTACACGGTTTAGTAGTTTAATTCATCGAATA100.11068068622025456No Hit
AGTCTCAATTGAGAATTCATTTGTTGGTAAGTGGAAACAATTATGTTTCC100.11068068622025456No Hit
AAGACTCCGAAGAGTATAAAACATCGCGTGCGCTTTACGACATCTGCGTT100.11068068622025456No Hit
TACTCAATCATACACATGACAACAAGTCATATTCGACTCCAAAACACTAA100.11068068622025456No Hit
GAATAGGTTTTAGTGGTTTCACTTTTCGTTGAGCTAGTGTATTAGTCCAT100.11068068622025456No Hit
GATGTTATAGGAAAATTGTATACGATGCAGAATTTCGTAATTTGTCCCAC100.11068068622025456No Hit
ACTATTTTGGTTTGAATTTTTTGAATCGTTTAGAGTTAGTATTATATATA100.11068068622025456No Hit
TCTAACCTGTTATCAAGGAGAATGCAAACAAGAGAGACAATGTTTGAGTT100.11068068622025456No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTAG401.10958354E-1070.000019
GGACTGT401.10958354E-1070.000017
GTATCAT207.509685E-570.0000163
CGGACTG453.6379788E-1270.000016
CGTATCA207.509685E-570.0000162
TGTAGAA401.10958354E-1070.0000111
GACTGTA401.10958354E-1070.000018
TAATAAT207.509685E-570.000016
CTGTAGA401.10958354E-1070.0000110
GAGAACA150.002153673470.049
TTCATCA150.002153673470.069
CGATATC150.002153673470.019
CCGGACT150.002153673470.05
TATATTC150.002153673470.037
TCGACGA150.002153673470.042
TGACTTA150.002153673470.032
ACATGGA150.002153673470.059
TCGATAT150.002153673470.018
GTCCGTA150.002153673470.029
AATCACC150.002153673470.010
Produced by FastQC (version 0.11.5)