FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_13YCol_14YC24_15YCol_16YC24_lib_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_13YCol_14YC24_15YCol_16YC24_lib_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153860
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAG795236.891910630405769Illumina Single End PCR Primer 1 (96% over 33bp)
ACTCAATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAG455203.9450193264347497Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG298932.590695578319727No Hit
ACTCTAATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCA65030.5635865702944898Illumina Single End PCR Primer 1 (96% over 33bp)
CTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGT45880.39762189520392416Illumina Single End PCR Primer 1 (96% over 33bp)
GGATCGATCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA33840.29327648068223183Illumina Single End PCR Primer 1 (96% over 33bp)
ATCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGT23870.20687085088312274Illumina Single End PCR Primer 1 (96% over 33bp)
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTATGGAAAGAGTGTGTCAG20880.18095782850605793Illumina Single End PCR Primer 1 (96% over 33bp)
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTCAG19510.16908463765101486Illumina Single End PCR Primer 1 (96% over 33bp)
GTGAGGTCTGAACTCCAGTCACCTCACAGATCGGAAGAGCGTCGTGTAGG17290.14984486852824433Illumina Single End PCR Primer 1 (100% over 23bp)
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTAAG15920.13797167767320126Illumina Single End PCR Primer 1 (96% over 33bp)
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAC13930.1207252179640511Illumina Single End PCR Primer 1 (96% over 33bp)
ACTCTATTAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCA13260.11491862097654827Illumina Single End PCR Primer 1 (96% over 33bp)
CACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAG12360.1071187145754251Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT159800.069.453351
ACTCTAT480500.069.359911
GTGAGGT146600.068.998171
CCGCATA69800.068.79152
ACCGCAT182150.068.502131
CCGCATT35650.068.031452
GATCGTA63500.067.459592
CTCTATC90650.067.408162
CTCTATG77900.067.299282
CCGCATG35300.067.218842
CGCATAT23500.067.165273
CTCTATT88250.066.98092
TGAGGTA64800.066.916372
CTCTATA241300.066.760522
GATCGTC37350.066.527892
TGAGGTC37450.066.44372
CGCATAC10800.066.430293
GATCGTG32950.065.958722
CTCAATA65300.065.4928742
TCAATAG64750.065.4546363
Produced by FastQC (version 0.11.5)