Basic Statistics
Measure | Value |
---|---|
Filename | HT5VLAFXY_n01_wg_dDAP_HALOLHY1_GSTCCA1_Col_B_k.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 22457444 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 39 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT | 511399 | 2.277191473793723 | TruSeq Adapter, Index 27 (97% over 39bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 161208 | 0.7178377022781399 | No Hit |
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC | 152559 | 0.6793248599439901 | TruSeq Adapter, Index 27 (97% over 36bp) |
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA | 61185 | 0.2724486366302416 | TruSeq Adapter, Index 27 (97% over 39bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGAGCTCGTAT | 24293 | 0.10817348581610624 | TruSeq Adapter, Index 27 (97% over 39bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AGGGGGG | 12895 | 0.0 | 46.649956 | 1 |
GCCGTCT | 88485 | 0.0 | 45.804016 | 51 |
TGCCGTC | 91155 | 0.0 | 45.64882 | 50 |
ATGCCGT | 91860 | 0.0 | 45.43945 | 49 |
TATGCCG | 92555 | 0.0 | 45.264626 | 48 |
GTATGCC | 93930 | 0.0 | 44.680267 | 47 |
CCGTCTT | 91495 | 0.0 | 44.33541 | 52 |
CGTATGC | 94970 | 0.0 | 44.11727 | 46 |
CTGCTTG | 88205 | 0.0 | 44.009556 | 59 |
CGTCTTC | 93235 | 0.0 | 43.294025 | 53 |
CTTCTGC | 92150 | 0.0 | 42.766895 | 56 |
TCGTATG | 97465 | 0.0 | 42.53904 | 45 |
CTCGTAT | 94040 | 0.0 | 41.959476 | 44 |
GCTTGAA | 94795 | 0.0 | 41.93599 | 61 |
TGCTTGA | 93495 | 0.0 | 41.66182 | 60 |
TTCTGCT | 92420 | 0.0 | 41.468433 | 57 |
TCTGCTT | 94640 | 0.0 | 41.18727 | 58 |
TCTCGTA | 94865 | 0.0 | 39.79782 | 43 |
AAGGGGG | 8820 | 0.0 | 39.761597 | 70 |
TCTTCTG | 100660 | 0.0 | 39.255608 | 55 |