FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_wg_dDAP_HALOLHY1_GSTCCA1_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_wg_dDAP_HALOLHY1_GSTCCA1_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22457444
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT5113992.277191473793723TruSeq Adapter, Index 27 (97% over 39bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1612080.7178377022781399No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC1525590.6793248599439901TruSeq Adapter, Index 27 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA611850.2724486366302416TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGAGCTCGTAT242930.10817348581610624TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG128950.046.6499561
GCCGTCT884850.045.80401651
TGCCGTC911550.045.6488250
ATGCCGT918600.045.4394549
TATGCCG925550.045.26462648
GTATGCC939300.044.68026747
CCGTCTT914950.044.3354152
CGTATGC949700.044.1172746
CTGCTTG882050.044.00955659
CGTCTTC932350.043.29402553
CTTCTGC921500.042.76689556
TCGTATG974650.042.5390445
CTCGTAT940400.041.95947644
GCTTGAA947950.041.9359961
TGCTTGA934950.041.6618260
TTCTGCT924200.041.46843357
TCTGCTT946400.041.1872758
TCTCGTA948650.039.7978243
AAGGGGG88200.039.76159770
TCTTCTG1006600.039.25560855