FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_wg_dDAP_HALOLHY1_GSTCCA1_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_wg_dDAP_HALOLHY1_GSTCCA1_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22457444
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT5113992.277191473793723TruSeq Adapter, Index 27 (97% over 39bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1612080.7178377022781399No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC1525590.6793248599439901TruSeq Adapter, Index 27 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA611850.2724486366302416TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGAGCTCGTAT242930.10817348581610624TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG130250.046.4808851
GCCGTCT882150.045.97198551
TGCCGTC908850.045.8152450
ATGCCGT917050.045.57731249
TATGCCG922600.045.46246748
GTATGCC935800.044.88851547
CCGTCTT911150.044.52415552
CGTATGC947600.044.2704546
CTGCTTG880000.044.17164259
CGTCTTC928350.043.4692653
CTTCTGC917550.042.92811656
TCGTATG971900.042.6702145
CTCGTAT935700.042.06924444
GCTTGAA947800.042.02008461
TGCTTGA934100.041.7558460
TTCTGCT922750.041.59419657
TCTGCTT944550.041.31601758
TCTCGTA943950.039.81059343
AAGGGGG87050.039.764270
TCTTCTG1003650.039.34657755