Basic Statistics
Measure | Value |
---|---|
Filename | HT5VLAFXY_n01_wg_dDAP_HALOLHY1_GSTCCA1_Col_B_k-b.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 22457444 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 39 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT | 511399 | 2.277191473793723 | TruSeq Adapter, Index 27 (97% over 39bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 161208 | 0.7178377022781399 | No Hit |
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC | 152559 | 0.6793248599439901 | TruSeq Adapter, Index 27 (97% over 36bp) |
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA | 61185 | 0.2724486366302416 | TruSeq Adapter, Index 27 (97% over 39bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGAGCTCGTAT | 24293 | 0.10817348581610624 | TruSeq Adapter, Index 27 (97% over 39bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AGGGGGG | 13025 | 0.0 | 46.480885 | 1 |
GCCGTCT | 88215 | 0.0 | 45.971985 | 51 |
TGCCGTC | 90885 | 0.0 | 45.81524 | 50 |
ATGCCGT | 91705 | 0.0 | 45.577312 | 49 |
TATGCCG | 92260 | 0.0 | 45.462467 | 48 |
GTATGCC | 93580 | 0.0 | 44.888515 | 47 |
CCGTCTT | 91115 | 0.0 | 44.524155 | 52 |
CGTATGC | 94760 | 0.0 | 44.27045 | 46 |
CTGCTTG | 88000 | 0.0 | 44.171642 | 59 |
CGTCTTC | 92835 | 0.0 | 43.46926 | 53 |
CTTCTGC | 91755 | 0.0 | 42.928116 | 56 |
TCGTATG | 97190 | 0.0 | 42.67021 | 45 |
CTCGTAT | 93570 | 0.0 | 42.069244 | 44 |
GCTTGAA | 94780 | 0.0 | 42.020084 | 61 |
TGCTTGA | 93410 | 0.0 | 41.75584 | 60 |
TTCTGCT | 92275 | 0.0 | 41.594196 | 57 |
TCTGCTT | 94455 | 0.0 | 41.316017 | 58 |
TCTCGTA | 94395 | 0.0 | 39.810593 | 43 |
AAGGGGG | 8705 | 0.0 | 39.7642 | 70 |
TCTTCTG | 100365 | 0.0 | 39.346577 | 55 |