FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_wg_dDAP_HALOCCA1_GSTLHY1_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_wg_dDAP_HALOCCA1_GSTLHY1_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8892518
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT2088662.348783550395962TruSeq Adapter, Index 27 (97% over 39bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG580090.6523349179613693No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC561160.6310473591394474TruSeq Adapter, Index 27 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA238030.2676744652077173TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGAGCTCGTAT95230.10709002781889225TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT357150.047.7148351
AGGGGGG54250.047.704631
TGCCGTC366500.047.37612550
ATGCCGT368550.047.16008849
TATGCCG371900.046.92349648
GTATGCC376650.046.378247
CCGTCTT370450.046.15292752
CGTATGC379000.046.0352246
CTGCTTG355750.045.44303559
CGTCTTC379450.044.77231253
TCGTATG387750.044.6353345
CTCGTAT373200.044.4529944
CTTCTGC374650.044.3089756
GCTTGAA384300.043.15080661
TGCTTGA378500.042.8873460
TTCTGCT376150.042.6993557
TCTCGTA373450.042.3332843
TCTGCTT384100.042.3167458
AAGGGGG34650.041.71681270
TCTTCTG408050.040.84512755
Produced by FastQC (version 0.11.5)