FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_wg_dDAP_HALOCCA1_GSTLHY1_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_wg_dDAP_HALOCCA1_GSTLHY1_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8892518
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT2088662.348783550395962TruSeq Adapter, Index 27 (97% over 39bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG580090.6523349179613693No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC561160.6310473591394474TruSeq Adapter, Index 27 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA238030.2676744652077173TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGAGCTCGTAT95230.10709002781889225TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT355850.047.8399551
TGCCGTC365200.047.4968350
ATGCCGT367450.047.27266749
AGGGGGG54900.047.204921
TATGCCG371100.047.04349548
GTATGCC375550.046.51402347
CCGTCTT369500.046.2810452
CGTATGC377850.046.18457446
CTGCTTG354950.045.53557259
CGTCTTC378250.044.92358453
TCGTATG386850.044.74820745
CTCGTAT372500.044.56469344
CTTCTGC373500.044.38915656
GCTTGAA383050.043.3007461
TGCTTGA377750.042.9446860
TTCTGCT374700.042.86456757
TCTCGTA372150.042.4623343
TCTGCTT383750.042.34619558
AAGGGGG35100.041.48110670
TCTTCTG406950.040.92971855
Produced by FastQC (version 0.11.5)