Basic Statistics
Measure | Value |
---|---|
Filename | HT5VLAFXY_n01_wg_HALOLHY1_pGSTCCA1_Col_B_k.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 3632428 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 39 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT | 115425 | 3.1776266453182282 | TruSeq Adapter, Index 27 (97% over 39bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 15790 | 0.4346954709081639 | No Hit |
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC | 12937 | 0.35615296435331956 | TruSeq Adapter, Index 27 (97% over 36bp) |
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA | 7869 | 0.2166319607711426 | TruSeq Adapter, Index 27 (97% over 39bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGAGCTCGTAT | 5443 | 0.14984467689380215 | TruSeq Adapter, Index 27 (97% over 39bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 16705 | 0.0 | 56.967594 | 50 |
TATGCCG | 16815 | 0.0 | 56.927956 | 48 |
GCCGTCT | 16415 | 0.0 | 56.886616 | 51 |
ATGCCGT | 16740 | 0.0 | 56.869396 | 49 |
GTATGCC | 17080 | 0.0 | 56.229134 | 47 |
CGTATGC | 17215 | 0.0 | 55.869507 | 46 |
CCGTCTT | 16860 | 0.0 | 55.468197 | 52 |
CTGCTTG | 16370 | 0.0 | 54.66976 | 59 |
TCGTATG | 17465 | 0.0 | 54.288216 | 45 |
CTCGTAT | 16850 | 0.0 | 53.797863 | 44 |
CGTCTTC | 17335 | 0.0 | 53.605064 | 53 |
CTTCTGC | 17095 | 0.0 | 53.354427 | 56 |
TGCTTGA | 17190 | 0.0 | 52.184063 | 60 |
GCTTGAA | 17655 | 0.0 | 51.840496 | 61 |
TCTCGTA | 16680 | 0.0 | 51.786224 | 43 |
TTCTGCT | 17155 | 0.0 | 51.596855 | 57 |
TCTGCTT | 17495 | 0.0 | 51.334324 | 58 |
GATCTCG | 16980 | 0.0 | 50.335354 | 41 |
TCTTCTG | 18390 | 0.0 | 49.844692 | 55 |
ATCTCGT | 17275 | 0.0 | 49.59735 | 42 |