FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_wg_HALOLHY1_pGSTCCA1_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_wg_HALOLHY1_pGSTCCA1_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3632428
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1154253.1776266453182282TruSeq Adapter, Index 27 (97% over 39bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG157900.4346954709081639No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC129370.35615296435331956TruSeq Adapter, Index 27 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA78690.2166319607711426TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGAGCTCGTAT54430.14984467689380215TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC167050.056.96759450
TATGCCG168150.056.92795648
GCCGTCT164150.056.88661651
ATGCCGT167400.056.86939649
GTATGCC170800.056.22913447
CGTATGC172150.055.86950746
CCGTCTT168600.055.46819752
CTGCTTG163700.054.6697659
TCGTATG174650.054.28821645
CTCGTAT168500.053.79786344
CGTCTTC173350.053.60506453
CTTCTGC170950.053.35442756
TGCTTGA171900.052.18406360
GCTTGAA176550.051.84049661
TCTCGTA166800.051.78622443
TTCTGCT171550.051.59685557
TCTGCTT174950.051.33432458
GATCTCG169800.050.33535441
TCTTCTG183900.049.84469255
ATCTCGT172750.049.5973542