HT5VLAFXY_n01_wg_HALOLHY1_pGSTCCA1_Col_B_k-b.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HT5VLAFXY_n01_wg_HALOLHY1_pGSTCCA1_Col_B_k-b.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3632428
Sequences flagged as poor quality	0
Sequence length	76
%GC	39

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT	115425	3.1776266453182282	TruSeq Adapter, Index 27 (97% over 39bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	15790	0.4346954709081639	No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC	12937	0.35615296435331956	TruSeq Adapter, Index 27 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA	7869	0.2166319607711426	TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGAGCTCGTAT	5443	0.14984467689380215	TruSeq Adapter, Index 27 (97% over 39bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	16760	56.968605	48
TGCCGTC	16660	56.953415	50
ATGCCGT	16690	56.89298	49
GCCGTCT	16375	56.875965	51
GTATGCC	17035	56.233856	47
CGTATGC	17185	55.86522	46
CCGTCTT	16805	55.503956	52
CTGCTTG	16335	54.701206	59
TCGTATG	17435	54.241116	45
CTCGTAT	16790	53.760822	44
CGTCTTC	17270	53.685234	53
CTTCTGC	17060	53.3408	56
TGCTTGA	17185	52.09742	60
TCTCGTA	16625	51.789135	43
GCTTGAA	17655	51.741383	61
TTCTGCT	17135	51.59581	57
TCTGCTT	17485	51.283627	58
GATCTCG	16965	50.23545	41
TCTTCTG	18320	49.92053	55
ATCTCGT	17245	49.521275	42