FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_wg_HALOCCA1_pGSTLHY1_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_wg_HALOCCA1_pGSTLHY1_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134356
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT506794.467645077912049TruSeq Adapter, Index 27 (97% over 39bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46720.4118636477437418No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC46300.4081611063898811TruSeq Adapter, Index 27 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA29420.2593542062632895TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGAGCTCGTAT23240.20487395491362498TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATGTCGTAT12700.11195779808102571TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT69900.058.48332651
TGCCGTC72050.058.146950
ATGCCGT72150.058.01779649
TATGCCG72100.057.96094548
GTATGCC73100.057.31168447
CCGTCTT71450.057.1656352
CGTATGC73750.056.7116546
CTGCTTG68800.056.56955359
CTTCTGC70900.055.9800456
CGTCTTC72750.055.95168353
TCGTATG74300.055.72657445
CTCGTAT70300.055.71102544
TGCTTGA70200.055.44138760
GCTTGAA71950.055.21174661
TTCTGCT70300.054.76508357
TCTCGTA69500.054.23720643
TCTGCTT71850.054.2169158
TCTTCTG74550.053.5678755
ATCTCGT71000.052.35191342
GATCTCG70750.052.2895641