Basic Statistics
Measure | Value |
---|---|
Filename | HT5VLAFXY_n01_wg_HALOCCA1_pGSTLHY1_Col_B_k.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1134356 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 39 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT | 50679 | 4.467645077912049 | TruSeq Adapter, Index 27 (97% over 39bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 4672 | 0.4118636477437418 | No Hit |
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC | 4630 | 0.4081611063898811 | TruSeq Adapter, Index 27 (97% over 36bp) |
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA | 2942 | 0.2593542062632895 | TruSeq Adapter, Index 27 (97% over 39bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGAGCTCGTAT | 2324 | 0.20487395491362498 | TruSeq Adapter, Index 27 (97% over 39bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATGTCGTAT | 1270 | 0.11195779808102571 | TruSeq Adapter, Index 27 (97% over 39bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCCGTCT | 6990 | 0.0 | 58.483326 | 51 |
TGCCGTC | 7205 | 0.0 | 58.1469 | 50 |
ATGCCGT | 7215 | 0.0 | 58.017796 | 49 |
TATGCCG | 7210 | 0.0 | 57.960945 | 48 |
GTATGCC | 7310 | 0.0 | 57.311684 | 47 |
CCGTCTT | 7145 | 0.0 | 57.16563 | 52 |
CGTATGC | 7375 | 0.0 | 56.71165 | 46 |
CTGCTTG | 6880 | 0.0 | 56.569553 | 59 |
CTTCTGC | 7090 | 0.0 | 55.98004 | 56 |
CGTCTTC | 7275 | 0.0 | 55.951683 | 53 |
TCGTATG | 7430 | 0.0 | 55.726574 | 45 |
CTCGTAT | 7030 | 0.0 | 55.711025 | 44 |
TGCTTGA | 7020 | 0.0 | 55.441387 | 60 |
GCTTGAA | 7195 | 0.0 | 55.211746 | 61 |
TTCTGCT | 7030 | 0.0 | 54.765083 | 57 |
TCTCGTA | 6950 | 0.0 | 54.237206 | 43 |
TCTGCTT | 7185 | 0.0 | 54.21691 | 58 |
TCTTCTG | 7455 | 0.0 | 53.56787 | 55 |
ATCTCGT | 7100 | 0.0 | 52.351913 | 42 |
GATCTCG | 7075 | 0.0 | 52.28956 | 41 |