HT5VLAFXY_n01_wg_HALOCCA1_pGSTLHY1_Col_B_k-b.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HT5VLAFXY_n01_wg_HALOCCA1_pGSTLHY1_Col_B_k-b.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1134356
Sequences flagged as poor quality	0
Sequence length	76
%GC	39

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT	50679	4.467645077912049	TruSeq Adapter, Index 27 (97% over 39bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	4672	0.4118636477437418	No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC	4630	0.4081611063898811	TruSeq Adapter, Index 27 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA	2942	0.2593542062632895	TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGAGCTCGTAT	2324	0.20487395491362498	TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATGTCGTAT	1270	0.11195779808102571	TruSeq Adapter, Index 27 (97% over 39bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCCGTCT	6985	58.625404	51
TGCCGTC	7200	58.2845	50
ATGCCGT	7210	58.155117	49
TATGCCG	7205	58.09832	48
GTATGCC	7305	57.446735	47
CCGTCTT	7135	57.34386	52
CGTATGC	7370	56.845104	46
CTGCTTG	6885	56.630142	59
CTTCTGC	7085	56.11835	56
CGTCTTC	7270	56.086445	53
TCGTATG	7430	55.820786	45
CTCGTAT	7040	55.731327	44
TGCTTGA	7025	55.501568	60
GCTTGAA	7200	55.270622	61
TTCTGCT	7035	54.82566	57
TCTGCTT	7190	54.27657	58
TCTCGTA	6960	54.25985	43
TCTTCTG	7445	53.733845	55
ATCTCGT	7110	52.37673	42
GATCTCG	7085	52.314552	41