FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23352500
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5837132.4995739214216894No Hit
ACTCTATAGAGTAGATCGGAAGACACACGTCTGAACTCCAGTCACGAGAT332490.14237876030403598TruSeq Adapter, Index 9 (96% over 25bp)
GTGAGGTGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCACAGATC257830.11040787924205117TruSeq Adapter, Index 1 (97% over 38bp)
ACTCTATAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA234380.10036612782357349TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT337800.067.711181
ACTCTAT637400.067.3535461
CTCTATA374050.065.499942
TGAGGTA166250.063.2485432
TATAGAG299200.062.085315
CCGCATT64550.061.8168952
GATCGTA146000.061.786962
TGAGGTC94750.060.0941122
TGAGTAG129600.059.8802038
GATCGTC77550.059.73742
GATCGTG81150.059.02322
GATCGTT82550.058.9102972
ATAGAGT297850.058.8387346
CGCATCG53550.058.151723
AGTAGAT424800.057.87211210
CGCATAG31400.057.813723
TAGAGTA300100.057.8031927
GCATGCG37850.057.3695834
CGCATAT39900.057.2218673
CGCATGC47800.056.674933
Produced by FastQC (version 0.11.5)