FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_dDAP_HALO_GST_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_dDAP_HALO_GST_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences895045
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT13340.149042785558268TruSeq Adapter, Index 13 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4000.036.7496248
TGCCGTC3850.035.45417450
ATGCCGT3950.035.4426749
CGTATGC4300.034.99963846
GCCGTCT3500.031.99966851
GTATGCC5050.029.10860847
TCGTATG5400.027.2219445
CTCGTAT5400.026.573844
CTGCGTG1251.0997428E-725.1997459
CCGTCTT5000.023.79975352
TGCGTGA1352.313609E-723.33309260
GCGTGAA1652.6593625E-923.3330961
CCCGCGG800.002666850321.87477366
TCTCGTA6950.020.64726643
ATCTCGT7700.019.5452542
AATCTCG7700.019.5452541
CTGCTTG5050.017.32655159
AGGGGGG1653.381644E-516.9771081
CGGACTG1452.4377157E-416.897326
CGTCTTC6500.016.69213553
Produced by FastQC (version 0.11.5)