FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_dDAP_HALOCOLSPL9_GSTC24SPL9_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_dDAP_HALOCOLSPL9_GSTC24SPL9_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences908647
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2250.024.88869350
CGGACTG1451.7285856E-824.1377416
TATGCCG2103.6379788E-1223.3331548
ATGCCGT2300.022.82590749
CGTATGC2301.2732926E-1121.30417846
GTATGCC2504.1836756E-1119.59984647
TGCCGGC900.005276270719.44429250
GCCGTCT2254.62569E-918.6665251
GCGCAAG950.007209795518.4209088
GGTGACC950.007209795518.42090812
CACACCC950.007209795518.42090868
TGTCCGT1000.00968929117.49986559
CCGGACT1350.00294612415.5554325
ACTGTAG2051.2585577E-515.3657339
GGACTGT1859.031629E-515.13501557
TCGTATG3707.021299E-1014.18907745
CTCGTAT3507.374183E-812.99989844
CCGTCTT3254.2321517E-712.92297652
CTGCTTG3407.3133197E-712.35284459
GACTGTA2305.71244E-412.1738178
Produced by FastQC (version 0.11.5)