FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_dDAP_HALOC24SPL9_GSTCOLSPL9_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_dDAP_HALOC24SPL9_GSTCOLSPL9_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17001199
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG37850.031.53209948
TGCCGTC36950.030.21625150
GCCGTCT34650.027.57552151
ATGCCGT43400.025.96752249
GTATGCC49100.024.80630747
CGTATGC57750.020.66649246
TCGTATG72900.015.939507545
CCGTCTT67050.015.39882652
CTCGTAT69650.015.17575244
CTGCGTG15500.014.90331859
CGTCTTC90150.011.60833253
CTGCTTG72800.011.20199259
ATCTCGT110050.010.46333742
AATCTCG111500.010.45282641
CTTCTGC93150.010.21998956
GCGTGAA31400.010.14337561
GCCGTCG20300.09.82750251
TGCGTGA23350.09.74310260
TCTCGTA116150.09.341289543
ATGCCGG15550.09.22821749
Produced by FastQC (version 0.11.5)