FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_dDAP_HALOC24SPL9_GSTCOLSPL9_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_dDAP_HALOC24SPL9_GSTCOLSPL9_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17001198
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG37600.031.7417548
TGCCGTC37250.029.40914750
GCCGTCT34000.028.10270351
ATGCCGT41350.026.66240949
GTATGCC50750.023.99979647
CGTATGC58700.020.39165146
CCGTCTT64100.015.889152
CTCGTAT68750.015.425323544
TCGTATG75350.014.81739345
CTGCGTG14300.013.70633859
CTGCTTG73450.011.67464759
CGTCTTC87900.011.30820853
CTTCTGC90450.010.75723456
AATCTCG113200.010.481359541
TGCCGGC16100.010.43469450
ATCTCGT112250.010.32062542
CGCGTAT14450.09.93071244
GCGTGAA29400.09.64288861
TGCGTGA23450.09.40301560
TCTCGTA119050.09.29013543
Produced by FastQC (version 0.11.5)