FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_bZIP63_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_bZIP63_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2486631
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG9700.024.89669242
TCGGGGG9050.021.27053543
CGGACTG3000.020.9998196
AATCGGG13000.019.11522141
CGGGGGG8800.017.89757344
CGGGGGT2903.43789E-1016.89640644
AGGGGGG4100.016.2291621
GAATCGG15450.015.85746940
GGACTGT4354.0017767E-1113.6780437
AGAATCG19100.013.37684739
GACTGTA4253.965397E-1013.1763588
TCCGGAG22750.011.84605234
CGGAGAA25400.011.71249836
CCGGAGA24300.011.66656635
GAGTCGC2100.004009585411.66656515
GGGGGGA4551.5341357E-811.53836262
CTCCGGA24400.011.4753133
ACTCCGG24000.011.2290732
CAGTCCG2903.2478318E-410.86197627
CACTCCG24950.010.8015131
Produced by FastQC (version 0.11.5)