FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_bZIP63_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_bZIP63_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2486631
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG9850.024.8728842
TCGGGGG9100.021.53827543
CGGACTG3000.020.9998196
AATCGGG13250.019.01870341
CGGGGGG8900.018.08973144
CGGGGGT2952.3646862E-1117.79645744
AGGGGGG4100.016.2291621
GAATCGG15650.015.87845840
AGAATCG19250.013.45442939
GGACTGT4455.820766E-1113.3706717
GACTGTA4355.7298166E-1012.8734528
TCCGGAG23150.011.94374234
CGGAGAA25750.011.82514136
CCGGAGA24650.011.78488835
CTCCGGA24700.011.61933233
GGGGGGA4601.8029823E-811.41294562
GAGTCGC2150.004779443611.3952515
ACTCCGG24300.011.37850232
CACTCCG25200.010.97212731
GGAGAAT27100.010.84861637
Produced by FastQC (version 0.11.5)