FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_bZIP53_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_bZIP53_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11206507
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG17350.010.08639842
CGGGGGG11400.09.5174944
TCCGGAG68000.07.72054570
TCGGGGG18500.07.189148443
CACTCCG83300.06.848700570
ACTCCGG76800.06.790326670
AATCGGG29750.06.352905841
CTCCGGA84200.06.19355269
GGCAGCG13406.6027496E-86.0097891
CCGGAGA79300.05.472857570
ACGCGGG14201.2836299E-65.4225052
CGGAGAA78900.05.411883470
CAGTCAC146450.05.305535370
TCCAGTC166350.05.281004470
CTCCAGT167750.05.153473469
GTCACTC124300.05.04019770
TCACTCC115400.05.004304469