FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_bZIP53_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_bZIP53_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11206507
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG17250.09.73907342
CGGGGGG11100.08.82877644
TCCGGAG68000.07.360250570
TCGGGGG17750.06.90136843
ACTCCGG75900.06.73250370
CACTCCG82350.06.63019870
GGCAGCG13301.2405508E-96.5816391
CTCCGGA83400.05.959197569
AATCGGG30400.05.75654541
CCGGAGA79550.05.587647469
CGGAGAA79300.05.56112770
CGCCCCG8200.0032043415.54874870
CGGGGGT8300.00369816465.481895470
TCCAGTC163350.05.42084370
CTCCAGT164650.05.293014569
CAGTCAC144200.05.291230770
CGCCCCC8800.0073522235.17042447