FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_SPL9_16YCol_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_SPL9_16YCol_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2649120
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT2551700.069.865161
GATCGTA1026950.069.597372
GATCGTC504200.069.242892
GATCGTG482100.069.0484852
GATCGTT563350.068.955782
ATCGTAA375000.068.5808643
ATCGTAG185750.068.096443
ATCGTAC138000.067.869113
ATCGTAT351150.067.846623
ATCGTGC60500.067.801193
ATCGTGT165550.067.716253
ATCGTGA172000.067.333853
ATCGTCA185300.067.279643
ATCGTCT179750.066.9623
ATCGTTA162000.066.758813
ATCGTGG96100.066.6852953
ATCGTTG139400.066.15813
TCGTAAA152400.065.911634
ATCGTCC90050.065.879623
ATCGTTT203800.065.431363
Produced by FastQC (version 0.11.5)