FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_SPL9_16YCol_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_SPL9_16YCol_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2649120
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT2551900.069.865171
GATCGTA1028100.069.601222
GATCGTC504500.069.243342
GATCGTG481400.069.039832
GATCGTT562650.068.9731452
ATCGTAA375150.068.60013
ATCGTAG186000.068.0801853
ATCGTAC138400.067.900553
ATCGTAT351550.067.81923
ATCGTGC60450.067.799383
ATCGTGT164800.067.6846163
ATCGTGA172100.067.3557363
ATCGTCA184700.067.213953
ATCGTCT180250.066.9510043
ATCGTTA161700.066.75283
ATCGTGG96050.066.683573
ATCGTTG139650.066.089783
TCGTAAA152850.065.992364
ATCGTCC90900.065.841143
ATCGTTT202950.065.48123
Produced by FastQC (version 0.11.5)