FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_SPL9_15YCol_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_SPL9_15YCol_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1693857
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCT1689309.973096902513022TruSeq Adapter, Index 2 (97% over 37bp)
ACTCTATTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCT706434.170540960659607TruSeq Adapter, Index 2 (97% over 37bp)
ACTCTATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTC291841.7229317468948087TruSeq Adapter, Index 2 (97% over 35bp)
ACTCTATTAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC149560.8829552907949136TruSeq Adapter, Index 11 (97% over 36bp)
ACTCTATAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTC77460.4572995241038647TruSeq Adapter, Index 2 (97% over 37bp)
ACTCTATAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTC47330.27942146237846527TruSeq Adapter, Index 2 (97% over 37bp)
ACTCTATAGAGTAGATCGAAGAGCACACGTCTGAACTCCAGTCACCGCTC44040.2599983351605242TruSeq Adapter, Index 2 (96% over 31bp)
ACTCTATATAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG42570.25131991661633774TruSeq Adapter, Index 2 (100% over 35bp)
ATAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTA35760.21111581438102509TruSeq Adapter, Index 2 (97% over 37bp)
ACTCTAATAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC34920.2061567180700614TruSeq Adapter, Index 11 (97% over 36bp)
ACTCTATAGAGTAGATCGGAAGACACACGTCTGAACTCCAGTCACCGCTC26670.15745130787309672TruSeq Adapter, Index 2 (96% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21680.1279919143115387No Hit
ACTCTATAGAGTAGATCGGAGAGCACACGTCTGAACTCCAGTCACCGCTC21510.126988287677177TruSeq Adapter, Index 2 (96% over 30bp)
ACTCTATAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCCCCGCT20460.12078941728847241TruSeq Adapter, Index 2 (97% over 35bp)
ACTCTATTGAGTAGATCGGAGAGCACACGTCTGAACTCCAGTCACCGCTC18040.10650249696402944TruSeq Adapter, Index 2 (96% over 30bp)
ACTCTATTGAGTAGATCGAAGAGCACACGTCTGAACTCCAGTCACCGCTC17460.1030783590350307TruSeq Adapter, Index 2 (96% over 31bp)
ATCTATAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTC17060.10071688460123848TruSeq Adapter, Index 2 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAT1616200.069.721031
CTCTATA704800.069.110722
CTCTATG214250.068.872442
CTCTATC292100.068.597712
CTCTATT436400.068.491842
TCTATAG331000.067.461863
TCTATTG163300.066.120273
CTATAGA280650.066.071264
TCTATCG75600.065.5088963
TCTATAA168700.065.456073
CTATCGG44500.065.201894
CTATTGA118850.064.963884
TCTATAC68200.064.816363
TGAGTAG90000.064.671878
TCTATCA91550.064.494463
TCTATGA80900.064.15913
TCTATAT171400.063.9144673
TCTATCC46000.063.836613
TATTGAG97750.063.769475
TCTATGC30050.063.5936623
Produced by FastQC (version 0.11.5)