FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_SPL9_15YCol_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_SPL9_15YCol_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1693857
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCT1689309.973096902513022TruSeq Adapter, Index 2 (97% over 37bp)
ACTCTATTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCT706434.170540960659607TruSeq Adapter, Index 2 (97% over 37bp)
ACTCTATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTC291841.7229317468948087TruSeq Adapter, Index 2 (97% over 35bp)
ACTCTATTAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC149560.8829552907949136TruSeq Adapter, Index 11 (97% over 36bp)
ACTCTATAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTC77460.4572995241038647TruSeq Adapter, Index 2 (97% over 37bp)
ACTCTATAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTC47330.27942146237846527TruSeq Adapter, Index 2 (97% over 37bp)
ACTCTATAGAGTAGATCGAAGAGCACACGTCTGAACTCCAGTCACCGCTC44040.2599983351605242TruSeq Adapter, Index 2 (96% over 31bp)
ACTCTATATAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG42570.25131991661633774TruSeq Adapter, Index 2 (100% over 35bp)
ATAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTA35760.21111581438102509TruSeq Adapter, Index 2 (97% over 37bp)
ACTCTAATAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC34920.2061567180700614TruSeq Adapter, Index 11 (97% over 36bp)
ACTCTATAGAGTAGATCGGAAGACACACGTCTGAACTCCAGTCACCGCTC26670.15745130787309672TruSeq Adapter, Index 2 (96% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21680.1279919143115387No Hit
ACTCTATAGAGTAGATCGGAGAGCACACGTCTGAACTCCAGTCACCGCTC21510.126988287677177TruSeq Adapter, Index 2 (96% over 30bp)
ACTCTATAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCCCCGCT20460.12078941728847241TruSeq Adapter, Index 2 (97% over 35bp)
ACTCTATTGAGTAGATCGGAGAGCACACGTCTGAACTCCAGTCACCGCTC18040.10650249696402944TruSeq Adapter, Index 2 (96% over 30bp)
ACTCTATTGAGTAGATCGAAGAGCACACGTCTGAACTCCAGTCACCGCTC17460.1030783590350307TruSeq Adapter, Index 2 (96% over 31bp)
ATCTATAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTC17060.10071688460123848TruSeq Adapter, Index 2 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAT1616050.069.7188341
CTCTATA704800.069.105752
CTCTATG213950.068.870862
CTCTATC292700.068.588622
CTCTATT436000.068.490452
TCTATAG330350.067.4886553
TCTATTG163450.066.1024253
CTATAGA280300.066.0913244
TCTATCG75600.065.55523
TCTATAA169350.065.494183
CTATCGG44600.065.212654
CTATTGA119050.064.942954
TCTATAC67900.064.845013
TGAGTAG90300.064.728338
TCTATCA91600.064.459253
TCTATGA80850.064.068913
TCTATAT171350.063.933123
TCTATCC46550.063.8342363
TATTGAG98100.063.7916955
TCTATTA115750.063.5892873
Produced by FastQC (version 0.11.5)