FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_SPL9_14YCol_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_SPL9_14YCol_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809284
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGGTCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTC76070.4204425618089808TruSeq Adapter, Index 2 (97% over 35bp)
CACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTC35310.1951600743719615TruSeq Adapter, Index 2 (97% over 37bp)
GTCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATT19080.10545608096904632TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGGT1703000.069.817461
TGAGGTA759100.069.5616762
TGAGGTC365250.069.2618562
TGAGGTG289650.069.105522
GAGGTAT257200.068.82943
GAGGTAA292350.068.658843
GAGGTAG143200.068.2643663
TGAGGTT307550.068.258522
GAGGTCT132450.068.176383
GAGGTCG48900.067.780893
GAGGTCA131900.067.691143
GAGGTAC82950.067.679033
GAGGTGT118150.067.4520953
GAGGTCC61850.066.943933
GAGGTGA104950.066.531393
GAGGTTA106600.066.191083
GAGGTTG82600.065.2963263
GAGGTGG54700.065.200813
AGGTATT91150.064.8159564
AGGTATA85300.064.7476654
Produced by FastQC (version 0.11.5)