FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_SPL9_14YCol_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_SPL9_14YCol_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809284
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGGTCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTC76070.4204425618089808TruSeq Adapter, Index 2 (97% over 35bp)
CACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTC35310.1951600743719615TruSeq Adapter, Index 2 (97% over 37bp)
GTCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATT19080.10545608096904632TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGGT1703150.069.815421
TGAGGTA758650.069.561422
TGAGGTC365100.069.261552
TGAGGTG289850.069.106132
GAGGTAT257000.068.814883
GAGGTAA292150.068.681893
TGAGGTT307900.068.29462
GAGGTAG143400.068.2667853
GAGGTCT132250.068.173623
GAGGTCG48950.067.7116553
GAGGTAC82700.067.672023
GAGGTCA131650.067.6601643
GAGGTGT117800.067.444533
GAGGTCC62150.067.014993
GAGGTGA105450.066.547843
GAGGTTA106500.066.2860953
GAGGTTG82750.065.389443
GAGGTGG54700.065.200813
AGGTAGA60550.064.79744
AGGTATA84900.064.722924
Produced by FastQC (version 0.11.5)