FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_SPL9_13YCol_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_SPL9_13YCol_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578265
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTC451597.809395346424217TruSeq Adapter, Index 2 (97% over 35bp)
ATGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTA29510.5103196631302258TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCG29280.5063422479313118TruSeq Adapter, Index 2 (96% over 32bp)
ACCGCATCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTC24640.4261022195706121TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTA19720.34102012053297365TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCAT14040.2427952582293585TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATGCGGTAGATCGAAGAGCACACGTCTGAACTCCAGTCACCGCTC12860.22238938894797367TruSeq Adapter, Index 2 (96% over 31bp)
ACCGCATGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTAT12120.20959248787320692TruSeq Adapter, Index 2 (97% over 37bp)
ACCGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCG11930.20630679705671276TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTC9340.1615176432950291TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATC7840.13557797895428567TruSeq Adapter, Index 2 (97% over 35bp)
ACCGCATTCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCT6800.11759314501137022TruSeq Adapter, Index 2 (97% over 35bp)
ACCGCATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCGC6740.11655555843774047TruSeq Adapter, Index 2 (97% over 35bp)
ACCGCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATT6030.10427745064978859TruSeq Adapter, Index 2 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCAT510750.069.835181
CCGCATA182100.069.8071062
CGCATAG37550.069.719683
CGCATAA61400.069.486283
CCGCATG88250.069.444072
CCGCATC152550.069.3339542
CCGCATT107850.069.1879962
GCATCGG57900.069.092584
CGCATCG67850.068.967633
CGCATCA33550.068.95613
CGCATAT58700.068.866453
CGCATAC26200.068.6634453
CGCATGT26400.068.408423
CATCGGA54900.067.895515
CGCATCT34550.067.871973
CGCATGA26150.067.8578343
CGCATTC20250.067.752423
CGCATTA29000.067.585543
GCCGCAT15350.067.537911
CGCATGG20400.067.2542343
Produced by FastQC (version 0.11.5)