FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_SPL9_13YCol_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_SPL9_13YCol_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578265
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTC451597.809395346424217TruSeq Adapter, Index 2 (97% over 35bp)
ATGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTA29510.5103196631302258TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCG29280.5063422479313118TruSeq Adapter, Index 2 (96% over 32bp)
ACCGCATCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTC24640.4261022195706121TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTA19720.34102012053297365TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCAT14040.2427952582293585TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATGCGGTAGATCGAAGAGCACACGTCTGAACTCCAGTCACCGCTC12860.22238938894797367TruSeq Adapter, Index 2 (96% over 31bp)
ACCGCATGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTAT12120.20959248787320692TruSeq Adapter, Index 2 (97% over 37bp)
ACCGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCG11930.20630679705671276TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTC9340.1615176432950291TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATC7840.13557797895428567TruSeq Adapter, Index 2 (97% over 35bp)
ACCGCATTCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCT6800.11759314501137022TruSeq Adapter, Index 2 (97% over 35bp)
ACCGCATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCGC6740.11655555843774047TruSeq Adapter, Index 2 (97% over 35bp)
ACCGCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATT6030.10427745064978859TruSeq Adapter, Index 2 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCAT510800.069.8352051
CCGCATA182150.069.807172
CGCATAG37600.069.720063
CGCATAA61450.069.4866943
CCGCATG88500.069.445642
CCGCATC152100.069.3552
CCGCATT107950.069.188762
GCATCGG57850.069.09184
CGCATCG67650.069.016323
CGCATCA33500.068.954543
CGCATAT58700.068.866453
CGCATAC26150.068.660893
CGCATGT26500.068.414413
CATCGGA54900.067.895515
CGCATCT34500.067.868893
CGCATGA26150.067.8578343
CGCATTC20350.067.763463
CGCATTA29150.067.597953
GCCGCAT15300.067.529711
CGCATGG20400.067.2542343
Produced by FastQC (version 0.11.5)