FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_SPL9_13YCol_14YCol_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_SPL9_13YCol_14YCol_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences702030
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTC208822.974516758543082TruSeq Adapter, Index 2 (97% over 35bp)
GTGAGGTCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTC21480.3059698303491304TruSeq Adapter, Index 2 (97% over 35bp)
ACCGCATGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCG15150.2158027434725012TruSeq Adapter, Index 2 (96% over 32bp)
ATGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTA11830.16851131718017748TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTC10430.148569149466547TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTA9090.1294816460835007TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGGT417950.069.748141
ACCGCAT249500.069.5193941
CCGCATA95200.069.448172
CCGCATT49700.069.3658452
TGAGGTA190150.069.171352
CCGCATG40750.069.140752
TGAGGTC92450.068.7881852
TGAGGTG69300.068.585512
CGCATAG21850.068.397823
CGCATAT32050.068.361593
CGCATAA30650.068.2867663
GCATCGG28350.068.1478044
CCGCATC74800.068.1282
CGCATCG34050.068.0466463
GAGGTAG36850.068.005083
GAGGTAT62750.067.9916843
GAGGTAC22150.067.945483
GAGGTAA73100.067.749313
GCCGCAT8100.067.431081
GAGGTGC6350.067.243753
Produced by FastQC (version 0.11.5)