FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_SPL9_13YCol_14YCol_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_SPL9_13YCol_14YCol_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences702030
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTC208822.974516758543082TruSeq Adapter, Index 2 (97% over 35bp)
GTGAGGTCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTC21480.3059698303491304TruSeq Adapter, Index 2 (97% over 35bp)
ACCGCATGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCG15150.2158027434725012TruSeq Adapter, Index 2 (96% over 32bp)
ATGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTA11830.16851131718017748TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTC10430.148569149466547TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTA9090.1294816460835007TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGGT418000.069.748171
ACCGCAT249400.069.519191
CCGCATT49650.069.435712
CCGCATA95250.069.411722
TGAGGTA190100.069.171142
CCGCATG40750.069.140752
TGAGGTC92550.068.78952
TGAGGTG69250.068.584492
CGCATAG21850.068.397823
CGCATAT32050.068.361593
CGCATAA30600.068.283973
GCATCGG28300.068.144534
CCGCATC74700.068.125492
CGCATCG34000.068.043773
GAGGTAG36750.067.999663
GAGGTAT62550.067.985273
GAGGTAC22200.067.95013
GCCGCAT8050.067.849911
GAGGTAA73300.067.755453
GAGGTGC6300.067.221883
Produced by FastQC (version 0.11.5)