FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_SPL9_13YCol_14YCol_15YCol_16YCol_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_SPL9_13YCol_14YCol_15YCol_16YCol_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2708341
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCT976473.6054174862028083TruSeq Adapter, Index 2 (97% over 37bp)
ACTCTATTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCT421681.5569679002754824TruSeq Adapter, Index 2 (97% over 37bp)
ACTCTATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTC208570.7701024354023367TruSeq Adapter, Index 2 (97% over 35bp)
ACCGCATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTC190700.7041210837187785TruSeq Adapter, Index 2 (97% over 35bp)
ACTCTATTAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC89240.3295006057213623TruSeq Adapter, Index 11 (97% over 36bp)
ACTCTATAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTC49650.18332255797922048TruSeq Adapter, Index 2 (97% over 37bp)
GTGAGGTCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTC34770.12838117504405833TruSeq Adapter, Index 2 (97% over 35bp)
ACTCTATAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTC30120.11121199287682017TruSeq Adapter, Index 2 (97% over 37bp)
ACTCTATAGAGTAGATCGAAGAGCACACGTCTGAACTCCAGTCACCGCTC29140.10759354158135923TruSeq Adapter, Index 2 (96% over 31bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT536500.069.525311
GTGAGGT555600.069.51761
ACTCTAT1354600.069.3571241
GATCGTA217550.068.294162
TGAGGTA247750.068.191242
CTCTATA568550.068.158872
CCGCATA67400.067.8185042
ACCGCAT193350.067.553231
GATCGTC108700.067.3270342
CTCTATC255800.067.2903752
CTCTATG203800.067.26892
CTCTATT371550.067.267722
TGAGGTC127900.067.235642
GATCGTT123100.066.7582552
GATCGTG104600.066.653442
TGAGGTG96950.066.569932
TCTATAG228550.066.002593
GAGGTAA97900.065.208943
CCGCATT38800.064.857782
GAGGTAT89100.064.775073
Produced by FastQC (version 0.11.5)