FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_SPL9_13YCol_14YCol_15YCol_16YCol_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_SPL9_13YCol_14YCol_15YCol_16YCol_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2708341
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCT976473.6054174862028083TruSeq Adapter, Index 2 (97% over 37bp)
ACTCTATTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCT421681.5569679002754824TruSeq Adapter, Index 2 (97% over 37bp)
ACTCTATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTC208570.7701024354023367TruSeq Adapter, Index 2 (97% over 35bp)
ACCGCATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTC190700.7041210837187785TruSeq Adapter, Index 2 (97% over 35bp)
ACTCTATTAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC89240.3295006057213623TruSeq Adapter, Index 11 (97% over 36bp)
ACTCTATAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTC49650.18332255797922048TruSeq Adapter, Index 2 (97% over 37bp)
GTGAGGTCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTC34770.12838117504405833TruSeq Adapter, Index 2 (97% over 35bp)
ACTCTATAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTC30120.11121199287682017TruSeq Adapter, Index 2 (97% over 37bp)
ACTCTATAGAGTAGATCGAAGAGCACACGTCTGAACTCCAGTCACCGCTC29140.10759354158135923TruSeq Adapter, Index 2 (96% over 31bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGGT556050.069.5243151
GGATCGT537050.069.519311
ACTCTAT1353850.069.356751
GATCGTA217700.068.279252
TGAGGTA247450.068.2031862
CTCTATA568450.068.12162
CCGCATA67100.067.75662
ACCGCAT193150.067.605051
GATCGTC109000.067.334382
CTCTATC255900.067.305112
CTCTATG203750.067.2682342
CTCTATT371300.067.2658842
TGAGGTC127900.067.263012
GATCGTT122800.066.7503362
GATCGTG104900.066.7297442
TGAGGTG97150.066.576982
TCTATAG228500.065.9864043
GAGGTAA97400.065.184343
CCGCATT38950.064.9674452
GAGGTAT89250.064.823073
Produced by FastQC (version 0.11.5)