FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_SPL9_13YCol_14YC24_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_SPL9_13YCol_14YC24_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315093
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTC660525.022610568225973TruSeq Adapter, Index 2 (97% over 35bp)
GTGAGGTCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTC113980.8667067652249689TruSeq Adapter, Index 2 (97% over 35bp)
GTGAGGTGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTAT61340.4664308911993297TruSeq Adapter, Index 2 (97% over 37bp)
ATGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTA43940.33412085685194887TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCG42760.325148107396207TruSeq Adapter, Index 2 (96% over 32bp)
CACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTC39030.29678509428610755TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTC35520.27009496666775656TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTA29020.22066880441155112TruSeq Adapter, Index 2 (97% over 37bp)
GTGAGGTGACTGGAGTTCAGACCTCACAGATCGGAAGAGCACACGTCTGA20480.15573043123185965Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCAT19130.1454649975324939TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATGCGGTAGATCGAAGAGCACACGTCTGAACTCCAGTCACCGCTC18580.14128278380312267TruSeq Adapter, Index 2 (96% over 31bp)
ACCGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCG17990.13679640907525173TruSeq Adapter, Index 2 (97% over 37bp)
GTCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATT17330.13177775260000624TruSeq Adapter, Index 2 (97% over 37bp)
GTGAGGTGACTGGAGTTCAGACCCTCACAGATCGGAAGAGCACACGTCTG16600.12622681437738623Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCATGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTAT16070.12219668114726487TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTC13850.1053157457305301TruSeq Adapter, Index 2 (97% over 37bp)
GTGAGGTGTCTGAACTCCAGTCACCTCACAGATCGAAGAGCACACGTCTG13450.10227413574553283TruSeq Adapter, Index 1 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCAT766600.069.769811
GTGAGGT436850.069.660351
CCGCATA273550.069.654242
CCGCATG135500.069.379772
CCGCATT152350.069.379412
CGCATAA89400.069.13843
GCATCGG88100.069.1256944
CGCATCG103550.068.8166963
CCGCATC235800.068.797422
CGCATAT90600.068.608973
TGAGGTA187300.068.598212
CGCATAG58150.068.555163
CGCATGG31350.068.436713
TGAGGTG89550.068.4363252
TGAGGTC104150.068.185012
CGCATGA39800.068.152973
CGCATCA54900.067.832133
CGCATGT42450.067.608663
CGCATAC40050.067.465383
CGCATTG36950.067.2527543
Produced by FastQC (version 0.11.5)