FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_SPL9_13YCol_14YC24_15YCOL_16YC24_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_SPL9_13YCol_14YC24_15YCOL_16YC24_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568567
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTC945456.0274760338576545TruSeq Adapter, Index 2 (97% over 35bp)
GGATCGATCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCA137650.8775525686821156TruSeq Adapter, Index 2 (97% over 37bp)
ATGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTA63330.40374430929631955TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCG58650.37390815948569617TruSeq Adapter, Index 2 (96% over 32bp)
ACCGCATCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTC52120.3322778051559162TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTA45720.2914762327653202TruSeq Adapter, Index 2 (97% over 37bp)
ACTCTATAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCT31060.19801513100811122TruSeq Adapter, Index 2 (97% over 37bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA28590.18226827416361557TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCAT27000.17213163352282687TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATGCGGTAGATCGAAGAGCACACGTCTGAACTCCAGTCACCGCTC26240.1672864468014436TruSeq Adapter, Index 2 (96% over 31bp)
ACCGCATGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTAT25060.15976365689192748TruSeq Adapter, Index 2 (97% over 37bp)
ACCGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCG22500.14344302793568908TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTC20270.12922623005584077TruSeq Adapter, Index 2 (97% over 37bp)
GGATCGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCAT18900.12049214346597882TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATC16540.10544656364694656TruSeq Adapter, Index 2 (97% over 35bp)
ACTCTATTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCT16370.10436277188032135TruSeq Adapter, Index 2 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCATA406950.069.784642
ACCGCAT1126850.069.7756351
GGATCGT290150.069.565661
CCGCATT230500.069.453022
CCGCATC338500.069.244862
CGCATAG81600.069.184713
CGCATAA130100.069.165693
CCGCATG192450.069.163072
CGCATAT140900.068.98123
GCATCGG126600.068.9491044
CGCATCG149950.068.9259643
CGCATAC58850.068.869663
GCCGCAT32050.068.167081
GATCGTA116250.068.102892
CGCATTA58450.067.9038543
CGCATTC43100.067.888293
CGCATCA78550.067.860893
CGCATGT60000.067.841333
GATCGTC60400.067.739732
CGCATTG58800.067.559193
Produced by FastQC (version 0.11.5)