FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_SPL9_13YCol_14YC24_15YCOL_16YC24_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_SPL9_13YCol_14YC24_15YCOL_16YC24_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568567
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTC945456.0274760338576545TruSeq Adapter, Index 2 (97% over 35bp)
GGATCGATCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCA137650.8775525686821156TruSeq Adapter, Index 2 (97% over 37bp)
ATGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTA63330.40374430929631955TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCG58650.37390815948569617TruSeq Adapter, Index 2 (96% over 32bp)
ACCGCATCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTC52120.3322778051559162TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTA45720.2914762327653202TruSeq Adapter, Index 2 (97% over 37bp)
ACTCTATAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCT31060.19801513100811122TruSeq Adapter, Index 2 (97% over 37bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA28590.18226827416361557TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCAT27000.17213163352282687TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATGCGGTAGATCGAAGAGCACACGTCTGAACTCCAGTCACCGCTC26240.1672864468014436TruSeq Adapter, Index 2 (96% over 31bp)
ACCGCATGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTAT25060.15976365689192748TruSeq Adapter, Index 2 (97% over 37bp)
ACCGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCG22500.14344302793568908TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTC20270.12922623005584077TruSeq Adapter, Index 2 (97% over 37bp)
GGATCGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCAT18900.12049214346597882TruSeq Adapter, Index 2 (97% over 37bp)
ACCGCATATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATC16540.10544656364694656TruSeq Adapter, Index 2 (97% over 35bp)
ACTCTATTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCT16370.10436277188032135TruSeq Adapter, Index 2 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCATA407050.069.784692
ACCGCAT1126950.069.775661
GGATCGT290000.069.565431
CCGCATT230950.069.438932
CCGCATC338200.069.254532
CGCATAG81750.069.1861953
CGCATAA130100.069.165693
CCGCATG192350.069.162642
CGCATAT140700.069.004623
GCATCGG126500.068.9482654
CGCATCG149750.068.924533
CGCATAC59000.068.813223
GCCGCAT32200.068.1757351
GATCGTA116300.068.13382
CGCATTA58600.067.968943
CGCATCA78750.067.910773
CGCATGT60000.067.841333
CGCATTC43300.067.8172153
GATCGTC60100.067.728452
CGCATTG58550.067.608543
Produced by FastQC (version 0.11.5)