FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_HALObZIP63_pGSTbZIP53_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_HALObZIP63_pGSTbZIP53_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644674
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG1502.910383E-1127.9999076
CTGAGCG500.00720817527.99990529
GGACTGT1455.9480953E-1026.5516387
ATCGGGG2259.094947E-1221.77770642
TCGGACT1301.056748E-418.8460925
CGGGGGT1000.0096860817.4999444
AATCGGG3002.910383E-1117.4999441
TCGGGGG2451.3251338E-817.14280143
GGAATGC1250.001761500316.7999448
GACTGTA1704.3811957E-516.4705338
GAATCGG3858.185452E-1114.54540540
ACTGTAG2052.1600969E-413.6584929
CCAGCTC2050.003343765211.9511870
GCTGACG2100.00399696511.6693431
CTGTAGA2459.700951E-411.42853410
AGCTCAG2200.00566458211.13632639
TCCGGAG5106.9612724E-910.98035534
AGAATCG4803.357127E-810.93746439
CTACACA2300.00788257510.65213935
TTGACAC2750.002541383510.18178550
Produced by FastQC (version 0.11.5)