FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_HALObZIP53_pGSTbZIP63_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_HALObZIP53_pGSTbZIP63_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11247735
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG13500.08.03696642
GCGGGCG13102.7094939E-75.8778117
CGCCCCG7950.00221866045.72327164
CGCGGAA11302.9548619E-55.57789951
ACTCCGG33750.05.392544770
CGGGGGT9909.299339E-45.302983844
TCGGGGG18501.0602889E-85.297250743
CACTCCG32650.05.145437269
ACGCCCC11006.8407325E-45.09093263
Produced by FastQC (version 0.11.5)