FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_HALObZIP53_pGSTbZIP63_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_HALObZIP53_pGSTbZIP63_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11247735
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG13751.8189894E-127.38175542
CGCCCCG7953.323622E-46.163524664
GCGGGCG13001.456865E-65.6537987
ACTCCGG33550.05.320371670
CGCGGAA11201.5384817E-45.31493571
CGGGGGT10150.0012967725.1723744
ACGCCCC10906.015472E-45.137639563
CGCGATC17801.3372482E-75.11231632
CACTCCG32450.05.06929469
Produced by FastQC (version 0.11.5)