FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_16YCol_Col_lib_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_16YCol_Col_lib_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6000310
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA124060.20675598427414582TruSeq Adapter, Index 7 (97% over 38bp)
GGATCGATCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATT68990.11497739283470353TruSeq Adapter, Index 7 (97% over 38bp)
GGATCGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC64940.10822774156668573TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT5835650.069.874771
GATCGTA2304800.069.5955662
GATCGTC1191050.069.244292
GATCGTT1224850.069.0736852
GATCGTG1173400.069.0480042
ATCGTAG460000.068.4245153
ATCGTAA831350.068.408133
ATCGTAC320400.067.945833
ATCGTAT744000.067.78853
ATCGTGC151900.067.603213
ATCGTGA420350.067.476623
ATCGTGT386400.067.291183
ATCGTCA436450.067.2088243
ATCGTGG245050.067.1143953
ATCGTCT403500.067.032983
ATCGTTA336150.066.230373
ATCGTCC217650.066.091883
ATCGTTG325150.066.027513
ATCGTTT427700.065.187753
TCGTAAA321650.065.0920264
Produced by FastQC (version 0.11.5)