FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_16YCol_Col_lib_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_16YCol_Col_lib_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6000310
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA124060.20675598427414582TruSeq Adapter, Index 7 (97% over 38bp)
GGATCGATCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATT68990.11497739283470353TruSeq Adapter, Index 7 (97% over 38bp)
GGATCGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC64940.10822774156668573TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT5835600.069.876571
GATCGTA2308100.069.594632
GATCGTC1189150.069.251912
GATCGTG1172500.069.053242
GATCGTT1224600.069.050632
ATCGTAA830500.068.4107063
ATCGTAG461100.068.390313
ATCGTAC321650.068.008223
ATCGTAT745750.067.803073
ATCGTGC152900.067.618873
ATCGTGA418200.067.497133
ATCGTGT386450.067.2824863
ATCGTCA436050.067.166133
ATCGTGG245050.067.157243
ATCGTCT402800.067.0278243
ATCGTTA336550.066.172463
ATCGTCC217300.065.972843
ATCGTTG324450.065.9434363
ATCGTTT426350.065.271023
TCGTAAA321900.065.139334
Produced by FastQC (version 0.11.5)