FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_15YCol_Col_lib_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_15YCol_Col_lib_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3150236
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA50074915.895602742143764TruSeq Adapter, Index 7 (97% over 37bp)
ACTCTATTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA31691410.060008202560063TruSeq Adapter, Index 7 (97% over 37bp)
ACTCTATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTC1901776.03691279002589TruSeq Adapter, Index 9 (97% over 35bp)
ACTCTATTAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG471841.497792546336211TruSeq Adapter, Index 7 (97% over 36bp)
ACTCTATAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT439401.3948161344102472TruSeq Adapter, Index 7 (97% over 38bp)
ACTCTATAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT261860.8312393103246867TruSeq Adapter, Index 7 (97% over 38bp)
ACTCTATAGAGTAGATCGAAGAGCACACGTCTGAACTCCAGTCACGAGAT239050.7588320367109004TruSeq Adapter, Index 9 (96% over 31bp)
ACTCTATAGAGTAGATCGGAGAGCACACGTCTGAACTCCAGTCACGAGAT143130.4543469124217995TruSeq Adapter, Index 7 (96% over 31bp)
ACTCTATTGAGTAGATCGGAGAGCACACGTCTGAACTCCAGTCACGAGAT134060.4255554187051383TruSeq Adapter, Index 7 (96% over 31bp)
ACTCTATTGAGTAGATCGAAGAGCACACGTCTGAACTCCAGTCACGAGAT109160.34651372151165816TruSeq Adapter, Index 9 (96% over 31bp)
ACTCTAATAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG87850.2788679959215754TruSeq Adapter, Index 7 (97% over 36bp)
ACTCTATAGAGTGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT87540.2778839426633433TruSeq Adapter, Index 7 (97% over 38bp)
ACTCTATATAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA87110.276518965563215TruSeq Adapter, Index 7 (97% over 35bp)
ACTCTATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAGCTC85070.270043260250978TruSeq Adapter, Index 9 (97% over 35bp)
ATAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCA81930.26007575305469177TruSeq Adapter, Index 7 (97% over 38bp)
ACTCTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCA76700.24347382227871184TruSeq Adapter, Index 7 (97% over 38bp)
ACTCTATTAAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA73660.23382375161733912TruSeq Adapter, Index 7 (97% over 37bp)
ACTCTATAGAGTAATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT68410.2171583335343765TruSeq Adapter, Index 9 (97% over 35bp)
ACTCTATAGAGTAGATGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT63590.20185789255154218TruSeq Adapter, Index 7 (97% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56450.17919292395871292No Hit
ACTCTATAGAGTAGATCGGAAGACACACGTCTGAACTCCAGTCACGAGAT51830.16452735604570579TruSeq Adapter, Index 9 (96% over 25bp)
ACTCTATAGAGTAGTCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT47840.15186163830265414TruSeq Adapter, Index 7 (97% over 36bp)
ATCTATAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT40380.12818087279810148TruSeq Adapter, Index 7 (97% over 38bp)
ACTCTATAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATT38570.12243527151616578TruSeq Adapter, Index 7 (97% over 38bp)
ACTCTATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATATC38170.12116552537651147TruSeq Adapter, Index 9 (97% over 35bp)
ACTCTATATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATC37100.11776895445293624TruSeq Adapter, Index 9 (97% over 35bp)
ACTCTATTGAGTAGATGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT36920.11719756869009179TruSeq Adapter, Index 7 (97% over 34bp)
ACTCTATGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCG35320.1121185841314746TruSeq Adapter, Index 7 (97% over 34bp)
ACTCTATAGAGAGATCGGAAGACACACGTCTGAACTCCAGTCACGAGATT35270.11195986586401781TruSeq Adapter, Index 9 (96% over 25bp)
ACTCTATTGAGTAGATCGGAAGACACACGTCTGAACTCCAGTCACGAGAT34120.10830934571251169TruSeq Adapter, Index 9 (96% over 25bp)
ACTCTATAGAGTAGATCGGAAGGCACACGTCTGAACTCCAGTCACGAGAT33700.1069761122658747TruSeq Adapter, Index 7 (96% over 28bp)
ACTCTATAGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT33640.10678565034492654TruSeq Adapter, Index 7 (97% over 38bp)
ACTCTATTGAGTAATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT32190.1021828205886797TruSeq Adapter, Index 9 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAT3038950.069.780911
CTCTATA1346550.069.34192
CTCTATT859600.069.266612
CTCTATC570400.069.0300142
CTCTATG301700.068.874222
TCTATTG491900.068.448393
TCTATAG891250.068.416913
CTATTGA437100.068.406074
CTATCGG252300.068.237734
TCTATCG291650.068.139413
TATTGAG409350.067.896195
CTATAGA833350.067.412384
TGAGTAG398700.067.330868
TATCGGA254700.066.3717355
TCTATAA192500.066.181353
TCTATTA175700.065.975633
TCTATAT215900.065.5900963
TCTATCC65550.065.514413
TCTATAC85650.065.341053
CTATTAG75650.065.141654
Produced by FastQC (version 0.11.5)