FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_14YCol_Col_lib_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_14YCol_Col_lib_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3344582
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGGTCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTC584251.7468550629047217TruSeq Adapter, Index 9 (97% over 35bp)
GTGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGT81960.2450530439977253TruSeq Adapter, Index 7 (97% over 38bp)
GTCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCC57000.17042488418582652TruSeq Adapter, Index 7 (97% over 38bp)
GTGAGGTCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG56790.16979700303356293TruSeq Adapter, Index 7 (97% over 36bp)
GTGAGGTGACTGGAGTTCAGACCTCACAGATCGGAAGAGCACACGTCTGA54060.16163454805413652Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTC41770.12488855109547323TruSeq Adapter, Index 7 (97% over 38bp)
GTGACCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATT40830.12207803546153151TruSeq Adapter, Index 7 (97% over 38bp)
GTGAAGGTCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCT40090.11986550187736465TruSeq Adapter, Index 9 (97% over 35bp)
GTGAGGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATT36860.11020809177350113TruSeq Adapter, Index 7 (97% over 38bp)
GTGAGGTGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAT35280.10548403358027998TruSeq Adapter, Index 7 (97% over 38bp)
GTGAGGTGACTGGAGTTCAGACCCTCACAGATCGGAAGAGCACACGTCTG35090.10491595063299389Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGGT3196050.069.848061
TGAGGTA1368350.069.505912
TGAGGTC752600.069.097372
TGAGGTG578550.068.8924942
GAGGTAT447900.068.74933
GAGGTAG274150.068.49313
GAGGTAA508500.068.437133
TGAGGTT540150.068.075122
GAGGTCT254350.067.921743
GAGGTAC164750.067.705193
GAGGTCG150900.067.610583
GAGGTCA248350.067.491033
GAGGTCC118450.067.222043
GAGGTGA211900.066.530973
GAGGTGT231000.066.499593
GAGGTTA179300.066.485923
GAGGTTG161850.065.8692253
GAGGTGC53850.065.6449053
GAGGTGG109150.064.9331743
AGGTAGA113200.064.774344
Produced by FastQC (version 0.11.5)