FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_14YCol_Col_lib_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_14YCol_Col_lib_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3344582
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGGTCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTC584251.7468550629047217TruSeq Adapter, Index 9 (97% over 35bp)
GTGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGT81960.2450530439977253TruSeq Adapter, Index 7 (97% over 38bp)
GTCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCC57000.17042488418582652TruSeq Adapter, Index 7 (97% over 38bp)
GTGAGGTCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG56790.16979700303356293TruSeq Adapter, Index 7 (97% over 36bp)
GTGAGGTGACTGGAGTTCAGACCTCACAGATCGGAAGAGCACACGTCTGA54060.16163454805413652Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTC41770.12488855109547323TruSeq Adapter, Index 7 (97% over 38bp)
GTGACCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATT40830.12207803546153151TruSeq Adapter, Index 7 (97% over 38bp)
GTGAAGGTCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCT40090.11986550187736465TruSeq Adapter, Index 9 (97% over 35bp)
GTGAGGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATT36860.11020809177350113TruSeq Adapter, Index 7 (97% over 38bp)
GTGAGGTGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAT35280.10548403358027998TruSeq Adapter, Index 7 (97% over 38bp)
GTGAGGTGACTGGAGTTCAGACCCTCACAGATCGGAAGAGCACACGTCTG35090.10491595063299389Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGGT3196450.069.849111
TGAGGTA1366750.069.50022
TGAGGTC753100.069.097952
TGAGGTG578350.068.867892
GAGGTAT445650.068.727263
GAGGTAG274150.068.556923
GAGGTAA508650.068.423823
TGAGGTT541550.068.138242
GAGGTCT254250.067.87963
GAGGTAC165100.067.7100453
GAGGTCG150750.067.631413
GAGGTCA249500.067.502573
GAGGTCC118150.067.2149663
GAGGTTA179600.066.5307543
GAGGTGT229700.066.525483
GAGGTGA212250.066.520193
GAGGTTG161750.065.88833
GAGGTGC53850.065.5798953
GAGGTGG109650.064.8924263
AGGTAGA112800.064.786824
Produced by FastQC (version 0.11.5)