FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_13YCol_Col_lib_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_13YCol_Col_lib_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1760927
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTC1623319.21849684853489TruSeq Adapter, Index 9 (97% over 35bp)
ACCGCATGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCG184791.049390463091315TruSeq Adapter, Index 9 (96% over 32bp)
ACCGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCG95940.5448266736781252TruSeq Adapter, Index 7 (97% over 38bp)
ACCGCATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAGCTC91680.5206348701564574TruSeq Adapter, Index 9 (97% over 35bp)
ACCGCATCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT82450.46821929585951033TruSeq Adapter, Index 7 (97% over 38bp)
ACCGCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCA76740.43579319301708697TruSeq Adapter, Index 7 (97% over 38bp)
ACCGCATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC47790.27139114795786534TruSeq Adapter, Index 7 (97% over 38bp)
ATGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCA40790.23163935813352854TruSeq Adapter, Index 7 (97% over 38bp)
ACCGCATGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT36210.20563032993417674TruSeq Adapter, Index 7 (97% over 38bp)
ACCGCATGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAT36030.2046081410529795TruSeq Adapter, Index 7 (97% over 38bp)
ACCGCATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATATC31350.17803123014185143TruSeq Adapter, Index 9 (97% over 35bp)
ACCGCATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCCTCTC29410.16701430553339236TruSeq Adapter, Index 9 (97% over 35bp)
ACCGCATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCGC25830.14668410445180294TruSeq Adapter, Index 9 (97% over 35bp)
ACCGCATGCGGTAGATCGAAGAGCACACGTCTGAACTCCAGTCACGAGAT24530.13930162919871183TruSeq Adapter, Index 9 (96% over 31bp)
ACCGCATTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT22980.1304994471661801TruSeq Adapter, Index 7 (97% over 38bp)
ACCGCATTCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCT22520.12788718669200938TruSeq Adapter, Index 7 (97% over 36bp)
ACCGCATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATGTC21080.11970967564243153TruSeq Adapter, Index 9 (97% over 35bp)
ACCGCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCC19910.11306544791464951TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCAT1670200.069.781541
CCGCATA562500.069.737992
CCGCATT322650.069.5111852
CCGCATG300150.069.5095752
CGCATAG126800.069.2816543
CGCATAA178900.069.177643
CGCATAT181550.069.112523
CCGCATC535650.069.019212
CGCATAC81150.068.834823
CGCATCG263550.068.591633
CGCATGG82300.068.5534063
CGCATGA89550.068.5532153
GCATCGG226050.068.543914
CGCATTG84900.068.144213
CGCATTA79400.068.015713
CGCATGT83100.067.809213
CGCATTC63750.067.748353
CGCATCT108450.067.514323
CATCGGA215000.067.459815
GCCGCAT35150.067.442991
Produced by FastQC (version 0.11.5)