FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_13YCol_14YCol_lib_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_13YCol_14YCol_lib_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2421772
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTC574782.3733860991042923TruSeq Adapter, Index 9 (97% over 35bp)
GTGAGGTCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTC94920.39194441095198057TruSeq Adapter, Index 9 (97% over 35bp)
ACCGCATGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCG57270.23647973467361916TruSeq Adapter, Index 9 (96% over 32bp)
ACCGCATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAGCTC34500.142457671490132TruSeq Adapter, Index 9 (97% over 35bp)
ACCGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCG32590.13457088445980875TruSeq Adapter, Index 7 (97% over 38bp)
ACCGCATCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT27300.11272737483132185TruSeq Adapter, Index 7 (97% over 38bp)
ACCGCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCA26280.10851558280465709TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGGT1536100.069.779241
ACCGCAT804900.069.445171
CCGCATA289600.069.443322
TGAGGTA669650.069.434792
TGAGGTC347100.068.930412
CCGCATT163150.068.797922
CCGCATG139400.068.7941062
TGAGGTG268450.068.669422
GAGGTAA253900.068.1796653
CCGCATC244450.068.109322
GAGGTAT225700.067.983333
CGCATAG64850.067.840453
GAGGTAG134800.067.610563
CGCATAA92000.067.602543
CGCATCG99750.067.437883
GAGGTCT130300.067.2863163
TGAGGTT274450.067.2829362
GCATCGG80500.067.17324
CGCATAC44650.067.098953
CGCATAT97000.067.040533
Produced by FastQC (version 0.11.5)