FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_13YCol_14YCol_lib_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_13YCol_14YCol_lib_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2421772
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTC574782.3733860991042923TruSeq Adapter, Index 9 (97% over 35bp)
GTGAGGTCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTC94920.39194441095198057TruSeq Adapter, Index 9 (97% over 35bp)
ACCGCATGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCG57270.23647973467361916TruSeq Adapter, Index 9 (96% over 32bp)
ACCGCATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAGCTC34500.142457671490132TruSeq Adapter, Index 9 (97% over 35bp)
ACCGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCG32590.13457088445980875TruSeq Adapter, Index 7 (97% over 38bp)
ACCGCATCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT27300.11272737483132185TruSeq Adapter, Index 7 (97% over 38bp)
ACCGCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCA26280.10851558280465709TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGGT1537000.069.785381
CCGCATA289050.069.454352
ACCGCAT803850.069.4501651
TGAGGTA671150.069.420382
TGAGGTC346400.068.938342
CCGCATT163550.068.8222352
CCGCATG139400.068.7689742
TGAGGTG269350.068.686832
GAGGTAA254700.068.212843
CCGCATC243750.068.132592
GAGGTAT226450.067.989983
CGCATAG65000.067.8454063
CGCATAA91750.067.672283
GAGGTAG134450.067.5782853
CGCATCG99650.067.470413
TGAGGTT273650.067.3133542
GAGGTCT130200.067.2573243
GCATCGG80500.067.173184
CGCATAC44600.067.095683
CGCATAT96500.067.061453
Produced by FastQC (version 0.11.5)